Phosphate-limited culture of Azotobacter vinelandii
نویسندگان
چکیده
منابع مشابه
Cytokinins in Azotobacter vinelandii Culture Medium.
Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. T...
متن کاملMetabolism of ribose-5-phosphate by Azotobacter vinelandii.
Because ribose-5-phosphate (R-5-P) and sedoheptulose-7-phosphate (S-7-P) have been found free in cells of the axotobacter, the mechanism of synthesis of these compounds was sought. Previously, Horecker (1) and Wood and Schwerdt (2) demonstrated in yeast and Pseudomonas Jluorestens that S-7-P was produced from R-5-P by an enzyme called transketolase. The R-5-P was first converted by an isomerase...
متن کاملSiderophores Produced by Nitrogen-Fixing Azotobacter vinelandii OP in Iron-Limited Continuous Culture.
Azotobacter vinelandii requires a high complement of iron and an efficient iron acquisition system to support nitrogen fixation. To circumvent problems inherent in batch culture trace metal studies, continuous cultures were used to measure the response of A. vinelandii to iron stress. Iron was found to be growth limiting for nitrogen-fixing A. vinelandii at a concentration as high as 12.5 muM; ...
متن کاملPlasmids of Azotobacter vinelandii.
Four laboratory strains and two isolates of Azotobacter vinelandii were found to contain plasmids. Twenty-five laboratory strains which could fix nitrogen did not have free, covalently closed circular plasmid DNA. The plasmids varied in size from 9 to 52 megadaltons, and each strain yielded only one plasmid. No discernible differences in ability to fix nitrogen were found between plasmid-bearin...
متن کاملUltrastructure of Azotobacter vinelandii.
Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not reported previously: intine fibrils, intine vesicles, intine membrane, and mi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1979
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.139.2.639-645.1979